MECP2 抗体 (pSer80)
Quick Overview for MECP2 抗体 (pSer80) (ABIN6656567)
抗原
See all MECP2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer80
-
Supplier Product No.
- 100-401-d71
-
Supplier
- Rockland
-
原理
- MeCP2 Phospho S80 Antibody
-
交叉反应 （详细）
- This antibody is specific for the ~75 kDa MECP2 protein phosphorylated at Ser 80 in Western blots of human, rat and mouse brain extracts.
-
纯化方法
- Anti-MeCP2 pS80 antibody was prepared from monospecific neat serum.
-
免疫原
- Anti-MeCP2 pS80 Antibody was produced in rabbit by repeated immunizations with MeCP Ser80 - Synthetic peptide corresponding to amino acid residues surrounding Ser 80 conjugated to KLH.
-
亚型
- IgG
-
-
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 7-148) antibody
MECP2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8H4A5B9 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-486) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (pSer423) antibodyMECP2 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB29261 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (C-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 400-428) antibodyMECP2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB25959 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4B6 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (N-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1:1000
-
说明
-
Anti-MeCP2 pS80 (Rabbit) has been tested in Western Blotting and IHC. Expect ~75kDa in WB. Anti-MeCP2 pS80 antibodies have been used successfully for IHS on mouse brain sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
-
别名
- MeCP2
-
背景
-
Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein
Background: Anti-MeCP2 pS80 antibody detects MeCP2 when phosphorylated at Ser80. MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors.
Gene Name: MECP2
-
基因ID
- 4204
-
UniProt
- P51608
-
途径
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
抗原
-
