Hsc70 抗体
(4 references)
Quick Overview for Hsc70 抗体 (ABIN6656068)
抗原
See all Hsc70 (HSPA8) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 200-301-a28
-
Supplier
- Rockland
-
原理
- Hsc70 (Hsp73) Antibody
-
交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Hsc70 from human, bovine, mouse, rat, C. Elegans
-
纯化方法
- This Protein G purified monoclonal antibody reacts with human Hsc70 (Hsp73) protein.
-
过滤
- Sterile filtered
-
免疫原
- This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein.
-
亚型
- IgG1
-
-
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibodyHSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibodyHSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) antibodyHSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibodyHSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibodyHSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (FITC)HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 FITC
-
-
应用备注
-
ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:200 - 1:1,000
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized
-
说明
-
This Protein G purified antibody has been tested for use in western blotting, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a doublet band approximately 72/73 kDa in size corresponding to Hsc70 (Hsp73) by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsc70 (Hsp73) in 20 μg of heat shocked HeLa cell lysate by western blotting.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
-
: "Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus." in: Frontiers in endocrinology, Vol. 12, pp. 627343, (2021) (PubMed).
: "TonEBP in dendritic cells mediates pro-inflammatory maturation and Th1/Th17 responses." in: Cell death & disease, Vol. 11, Issue 6, pp. 421, (2021) (PubMed).
: "TonEBP/NFAT5 promotes obesity and insulin resistance by epigenetic suppression of white adipose tissue beiging." in: Nature communications, Vol. 10, Issue 1, pp. 3536, (2019) (PubMed).
: "LPS-induced NFκB enhanceosome requires TonEBP/NFAT5 without DNA binding." in: Scientific reports, Vol. 6, pp. 24921, (2018) (PubMed).
-
: "Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus." in: Frontiers in endocrinology, Vol. 12, pp. 627343, (2021) (PubMed).
-
- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
-
别名
- Hsc70 Hsp73
-
背景
-
Synonyms: Heat shock cognate protein 71- kDa antibody, Heat shock protein 8 antibody, Heat-shock70-KD protein 10, formerly antibody, HSC54 antibody, HSC71 antibody
Background: Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.
Gene Name: HSPA1A
-
基因ID
- 3312
-
NCBI登录号
- NP_006588
-
UniProt
- P11142
抗原
-
