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NeuN 抗体 (N-Term)

This 小鼠 单克隆 antibody specifically detects NeuN in WB, IHC, ELISA 和 FM. It exhibits reactivity toward 人.It has been mentioned in 1 publication
Rockland
产品编号 ABIN6656067
Supplier Product No.: 200-301-x09
发货至: 中国
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Quick Overview for NeuN 抗体 (N-Term) (ABIN6656067)

抗原

See all NeuN (RBFOX3) 抗体
NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

适用

  • 66
  • 57
  • 54
  • 16
  • 4
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  • 1
  • 1
  • 1

宿主

  • 57
  • 14
  • 1
小鼠

克隆类型

  • 36
  • 36
单克隆

标记

  • 26
  • 5
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
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This NeuN antibody is un-conjugated

应用范围

  • 46
  • 27
  • 24
  • 21
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  • 15
  • 14
  • 13
  • 6
  • 5
  • 3
  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

克隆位点

1B7
  • 抗原表位

    • 16
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Supplier Product No.

    200-301-x09

    Supplier

    Rockland

    原理

    FOX3 (NeuN) Antibody

    纯化方法

    Anti-FOX3 Antibody was purified from tissue culture supernatant by protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    Anti-FOX3 Antibody was produced in mice by repeated immunizations with a recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.

    亚型

    IgG2a
  • 应用备注

    ELISA_Dilution: 1:20,000

    Immunohistochemistry_Dilution: 1:200-1:1000

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:2000

    Other: ICC: 1:200-1:1000

    说明

    Suggested Applications: Other
    Anti-FOX3 Antibody is tested for Western Blot, immunohistochemistry, and immunocytochemistry, and it is specific for the ~46/48 kDa FOX3 protein doublet. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage upon thawing. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

    有效期

    12 months
  • Zhou, Wang, Zha: "RNA-Seq analysis of knocking out the neuroprotective proton-sensitive GPR68 on basal and acute ischemia-induced transcriptome changes and signaling in mouse brain." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 35, Issue 4, pp. e21461, (2021) (PubMed).

  • 抗原

    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

    别名

    FOX3 (NeuN)

    背景

    Synonyms: RNA binding protein fox-1 homolog 3, Fox-1 homolg C, NeuN, hexaribonucleotide binding protein 3

    Background: FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing. FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development. FOX3 Antibody is ideal for researchers interested in neuroscience research.

    Gene Name: RBFOX3

    基因ID

    146713

    NCBI登录号

    NP_001076044

    UniProt

    A6NFN3
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