ENO2/NSE 抗体
Quick Overview for ENO2/NSE 抗体 (ABIN6656045)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-e00
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Supplier
- Rockland
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原理
- Neuron specific enolase (NSE) Antibody
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交叉反应 （详细）
- This antibody is a monospecific neat serum.
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纯化方法
- Anti-NSE antibody directed against human neuron specific enolase protein.
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免疫原
- Anti-NSE Antibody was produced by repeated immunizations with recombinant human NSE expressed in E. coli.
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亚型
- IgG
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibody
ENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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IF_Microscopy_Dilution: 1:500
Western_Blot_Dilution: 1:2000
Other: User Optimized
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说明
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Anti-Neuron specific enolase (Rabbit) antibody has been tested by Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in size corresponding to NSE protein by western blotting.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- Neuron specific enolase (NSE)
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背景
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Synonyms: Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, NSE
Background: NSE antibody detects NSE protein. Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. Anti-neuron specific enolase (NSE) antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.
Gene Name: ENO2
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基因ID
- 2026
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UniProt
- P09104
抗原
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