DNMT1 抗体
Quick Overview for DNMT1 抗体 (ABIN6655855)
抗原
See all DNMT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-h69
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Supplier
- Rockland
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原理
- DNMT1 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Dnmt1 from human, mouse and Zebrafish based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Dnmt1 Antibody was purified by Protein G chromatography.
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免疫原
- Dnmt1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with Dnmt1 human protein.
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亚型
- IgG1 kappa
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anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-270) antibody
DNMT1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibodyDNMT1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibodyKO Validated DNMT1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1448-1594) antibodyDNMT1 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C11A2 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 21-120) antibodyDNMT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-270) antibodyDNMT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1317-1616) antibodyDNMT1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2D10G8 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 881-914) antibodyDNMT1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB51461 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibodyDNMT1 适用: 人 WB, IHC, IP, ChIP 宿主: 小鼠 Monoclonal 60B1220-1 unconjugated
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1317-1616) antibodyDNMT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5G2 unconjugated
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应用备注
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ELISA_Dilution: 0.1-0.5 μg/mL
Immunohistochemistry_Dilution: 1-2 μg/mL
Flow_Cytometry_Dilution: 1-5 μg/mL
Western_Blot_Dilution: 0.1-0.5 μg/mL
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说明
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Anti-DNMT1 antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, and IP. Expect a band approximately 183 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
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别名
- Dnmt1
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背景
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Synonyms: AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI
Background: Anti-Dnmt1 antibody detects human Dnmt1. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Anti-Dnmt1 antibody is ideal for investigators involved in cancer, cell cycle protein research and epigenetics.
Gene Name: DNMT1
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基因ID
- 1786
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NCBI登录号
- NP_001124295
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UniProt
- P26358
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途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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