TNFAIP3 抗体 (C-Term)
Quick Overview for TNFAIP3 抗体 (C-Term) (ABIN6655643)
抗原
See all TNFAIP3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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Supplier Product No.
- 200-301-h52
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Supplier
- Rockland
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原理
- A20 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with Anti-A20 from Human, Mouse and rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-A20 Antibody was purified by Protein G chromatography.
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免疫原
- A20 Antibody was produced in mice by repeated immunizations with recombinant human A20 protein at the c-terminus.
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亚型
- IgG1 kappa
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应用备注
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Immunoprecipitation_Dilution: 1-2 μg/mL
Western_Blot_Dilution: 2-4 μg/mL
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说明
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Anti-A20 antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))
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别名
- TNFAIP3
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背景
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Synonyms: OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, A20p50, A20p37
Background: Anti-A20 antibody detects human A20. A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase. The A20 monoclonal antibody has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology. Anti-A20 antibody is ideal for investigators involved in NFkappaB and Signaling research.
Gene Name: TNFAIP3
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基因ID
- 7128
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NCBI登录号
- NP_001257436
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UniProt
- P21580
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response
抗原
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