CD4 抗体 (APC)
Quick Overview for CD4 抗体 (APC) (ABIN6655530)
抗原
See all CD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 200-326-n18
-
Supplier
- Rockland
-
原理
- CD4 Allophycocyanin Antibody
-
交叉反应 （详细）
- Reactivity is observed against human CD4, Cynomolgus, Rhesus, and chimpanzee.
-
纯化方法
- Allophycocyanin conjugated CD4 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD4.
-
过滤
- Sterile filtered
-
免疫原
- Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with CD4 antigen.
-
亚型
- IgG2b kappa
-
Labeling Ratio
- 1-2
-
-
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
anti-CD4 (CD4) (AA 26-200) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: User Optimized
-
说明
-
Anti-CD4 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunofluorescence, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
-
有效期
- 6 months
-
-
- CD4
-
别名
- CD4
-
背景
-
Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, APC, T4
Background: The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression.
Gene Name: CD4
-
基因ID
- 920
-
NCBI登录号
- NP_000607
-
UniProt
- P01730
-
途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
-
