Caspase 3 抗体
Quick Overview for Caspase 3 抗体 (ABIN6655470)
抗原
See all Caspase 3 (CASP3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-h63
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Supplier
- Rockland
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原理
- Caspase-3 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-3 from Human, mouse and rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Caspase-3 Antibody was purified by Protein G chromatography.
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免疫原
- Caspase-3 Antibody was produced in mice by repeated immunizations with a full length recombinant Caspase-3 human protein.
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亚型
- IgG1 kappa
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anti-Caspase 3 (CASP3) (AA 60-100) antibody
CASP3 适用: 人, 小鼠, 大鼠, Cow, 山羊 WB, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 67-175) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 220-236), (C-Term) antibodyCASP3 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 11-120) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal 5E1 unconjugated
anti-Caspase 3 (CASP3) (cleaved) antibodyCASP3 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (Internal Region) antibodyCASP3 适用: 人, 小鼠, 大鼠, Cow WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (pSer150) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 29-175) antibodyCASP3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 29-175) antibodyCASP3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
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应用备注
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Immunohistochemistry_Dilution: 5 μg/mL
Western_Blot_Dilution: 1-5 μg/mL
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说明
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Anti-Caspase 3 Antibody is tested for use in WB, EM, Flow, ICC/IF, IHC-Fr, and IHC-P. Expect a band approximately 31.5 kDa on specific lysates. The antibody detects both pro Caspase-3(32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Caspase 3 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Caspase 3 (CASP3)
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别名
- Caspase-3
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背景
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Synonyms: Cpp32, Caspase-3 subunit p17, Caspase-3 subunit p12, Apopain, Cysteine protease CPP32 Caspase-3, CASP-3, 3.4.22.56, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12
Background: Anti-Caspase-3 antibody detects human Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein. Caspase-3 cleaves the death substrate poly (ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Anti-Caapase-3 is ideal for investigators involved in apoptosis, cytokines and growth factor research.
Gene Name: Casp3
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基因ID
- 836
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NCBI登录号
- NP_004337
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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