PLK1 抗体
Quick Overview for PLK1 抗体 (ABIN6655435)
抗原
See all PLK1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-901-mh7s
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Supplier
- Rockland
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原理
- PLK1 Antibody
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交叉反应 （详细）
- Reactivity occurs against human PLK1.
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纯化方法
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
- Anti-PLK1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C1 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬, 猴 WB, IF, IP, FACS 宿主: 小鼠 Monoclonal 8C12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibodyPLK1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibodyPLK1 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 1D1 unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: 5 μg/mL
Western_Blot_Dilution: 1:500
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说明
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Anti-PLK1 antibody has been tested in Western Blot and Immunofluorescence. Expect a band ~68.3kDa in appropriate cell lysates or tissues. Positive controls used are HEK293T or K-562 whole cell lysate in WB, and HeLa cells in IF. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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别名
- PLK1
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背景
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Synonyms: Chicken Anti-Polo Like Kinase 1 Antibody, Chicken Anti-PLK1 Antibody, Chicken PLK-1 Antibody, Serine/Threonine-Protein Kinase 13, Polo-Like Kinase 1, STPK13, PLK-1, PLK, Serine/Threonine-Protein Kinase PLK1, Cell Cycle Regulated Protein Kinase, Polo-Like Kinase (Drosophila), Polo (Drosophila)-Like Kinase, EC 2.7.11.21
Background: Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that belongs to the CDC5/Polo subfamily and are critical regulators of cell cycle progression, mitosis, cytokinesis, elevated levels are found in many different types of cancer, and the DNA damage response. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis, hence, it is a target for cancer therapy. PLK-1, -2 and -3 are ubiquitously expressed, whereas PLK-4 is restricted to a few tissues. Diseases associated with PLK1 include Breast Cancer, Transient Tic Disorder, and Monoclonal Paraproteinemia. Anti-PLK1 Antibody is useful for researchers interested in Cancer Researcher, Cell Cycle Research, and Kinase Activity Research.
Gene Name: PLK1
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基因ID
- 5347
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NCBI登录号
- NP_005021
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UniProt
- P53350
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途径
- Cell Division Cycle, M Phase
抗原
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