DDIT4 抗体 (pThr23, pThr25)
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- 抗原 See all DDIT4 抗体
- DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))
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抗原表位
- pThr23, pThr25
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DDIT4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated REDD1. Reactivity against REDD1 occurs from human sources. However, reactivity is also expected against non-human primate based on 100% sequence homology.
- 免疫原
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Immunogen: Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product DDIT4 Primary Antibody
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anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 20-49), (N-Term) antibody
DDIT4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB12592 unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 1-232) antibodyDDIT4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 198-227), (C-Term) antibodyDDIT4 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB11618 unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (N-Term) antibodyDDIT4 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 1-232) antibodyDDIT4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 45-94) antibodyDDIT4 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (pThr23), (pThr25) antibodyDDIT4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (C-Term), (N-Term) antibodyDDIT4 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Phospho-Thr23/25 REDD1 Antibody is suitable for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:5,000
Western Blot Dilution: 1:500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- RT,-20 °C
- 储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut
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- 抗原
- DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))
- 别名
- REDD1 (DDIT4 产品)
- 别名
- redd1 antibody, Dig2 antibody, REDD-1 antibody, REDD1 antibody, 5830413E08Rik antibody, AA415483 antibody, Rtp801 antibody, dig2 antibody, rtp801 antibody, sb:cb821 antibody, zgc:64114 antibody, DNA damage inducible transcript 4 antibody, DNA-damage-inducible transcript 4 antibody, DNA damage inducible transcript 4 S homeolog antibody, ddit4 antibody, DDIT4 antibody, Ddit4 antibody, ddit4.S antibody
- 背景
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Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1
Background: REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.
Gene Name: DDIT4
- 基因ID
- 54541
- NCBI登录号
- NP_061931
- UniProt
- Q9NX09
- 途径
- Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process
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