DDIT4 抗体 (pThr23, pThr25)
Quick Overview for DDIT4 抗体 (pThr23, pThr25) (ABIN6655385)
抗原
See all DDIT4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr23, pThr25
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Supplier Product No.
- 600-401-x24
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Supplier
- Rockland
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原理
- REDD1 Phospho T23/25 Antibody
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交叉反应 （详细）
- Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.
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亚型
- IgG
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anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 20-49), (N-Term) antibody
DDIT4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB12592 unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 1-232) antibodyDDIT4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 198-227), (C-Term) antibodyDDIT4 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB11618 unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 1-232) antibodyDDIT4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (N-Term) antibodyDDIT4 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (AA 45-94) antibodyDDIT4 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (pThr23), (pThr25) antibodyDDIT4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 4 (DDIT4) (C-Term), (N-Term) antibodyDDIT4 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:5,000
Western_Blot_Dilution: 1:500
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说明
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Anti-Phospho-Thr23/25 REDD1 Antibody is tested for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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有效期
- 12 months
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- DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))
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别名
- REDD1
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背景
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Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1
Background: REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.
Gene Name: DDIT4
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基因ID
- 54541
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NCBI登录号
- NP_061931
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UniProt
- Q9NX09
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途径
- Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process
抗原
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