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CD20 抗体 (PE)

This anti-CD20 antibody is a 小鼠 单克隆 antibody detecting CD20 in FACS, IHC 和 IP. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
Rockland
产品编号 ABIN6655314
Supplier Product No.: 200-308-n64
发货至: 中国

Quick Overview for CD20 抗体 (PE) (ABIN6655314)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD20 antibody is conjugated to PE

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

克隆位点

2H7
  • Supplier Product No.

    200-308-n64

    Supplier

    Rockland

    原理

    CD20 Phycoerythrin Antibody

    交叉反应 (详细)

    Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey.

    纯化方法

    Phycoerythrin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20.

    过滤

    Sterile filtered

    免疫原

    Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.

    亚型

    IgG2b kappa

    Labeling Ratio

    1-2
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD20 is tested for Flow Cytometry and is useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • Tedder, Engel: "CD20: a regulator of cell-cycle progression of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 450-4, (1994) (PubMed).

    Hultin, Hausner, Hultin, Giorgi: "CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes." in: Cytometry, Vol. 14, Issue 2, pp. 196-204, (1993) (PubMed).

  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    CD20

    背景

    Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

    Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

    Gene Name: MS4A1

    基因ID

    931

    NCBI登录号

    NP_068769

    UniProt

    P11836
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