CD20 抗体 (APC)
Quick Overview for CD20 抗体 (APC) (ABIN6655312)
抗原
See all CD20 (MS4A1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-326-n64
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Supplier
- Rockland
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原理
- CD20 Allophycocyanin Antibody
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交叉反应 (详细)
- Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey.
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纯化方法
- Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20.
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过滤
- Sterile filtered
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免疫原
- Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.
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亚型
- IgG2b kappa
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Labeling Ratio
- 1-2
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
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说明
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Anti-CD20 is tested for Flow Cytometry and useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- CD20
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背景
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Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20
Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Gene Name: MS4A1
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基因ID
- 931
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NCBI登录号
- NP_068769
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UniProt
- P11836
抗原
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