CD11b 抗体 (FITC)
Quick Overview for CD11b 抗体 (FITC) (ABIN6655310)
抗原
See all CD11b (ITGAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-302-n20
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Supplier
- Rockland
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原理
- CD11b Fluorescein Antibody
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交叉反应 (详细)
- Reactivity is observed against human CD11b, Chimpanzee, Baboon, Cynomolgus, Rhesus, Common Marmoset, and Swine.
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纯化方法
- Fluorescein conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b.
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过滤
- Sterile filtered
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免疫原
- Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.
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亚型
- IgG1 kappa
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Labeling Ratio
- 4-6
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
IF_Microscopy_Dilution: User Optimized
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说明
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Anti-CD11b has been tested in Flow Cytometry and is useful for Immunofluorescence and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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别名
- CD11b
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背景
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Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A
Background: CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and Factor X.
Gene Name: ITGAM
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基因ID
- 3684
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NCBI登录号
- NP_000623
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UniProt
- P11215
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途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
抗原
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