Histone H2B 抗体 (C-Term)
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Quick Overview for Histone H2B 抗体 (C-Term) (ABIN6655251)
抗原
See all Histone H2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-x47
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Supplier
- Rockland
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原理
- Histone H2B pan Antibody
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交叉反应 （详细）
- Cross reactivity with other species was not tested.
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纯化方法
- Anti-Histone H2B pan Antibody was purified by affinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-Histone H2B pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the C-terminus of histone H4.
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亚型
- IgG
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anti-Histone H2B (acLys15) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys12) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys12) antibody适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK20ac) antibody适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人 WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Histone H2B (AA 1-126) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys120) antibody适用: 人 WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A6 unconjugated
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应用备注
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ChIP_Dilution: 2 μg per IP
ELISA_Dilution: 1:1000 - 1:5000
IF_Microscopy_Dilution: 1:1,000
Western_Blot_Dilution: 1:2,000 - 1:10,000
Other: Dot Blot: 1:20,000
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说明
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Anti-Histone H2B pan Antibody has been tested by Chromatin Immunoprecipitation, western blot, Immunofluorescence, and ELISA. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300 -
储存液
- ProClin, Sodium azide
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注意事项
- This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Histone H2B
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背景
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Synonyms: Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l
Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H2B pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.
Gene Name: HIST1H2BC
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基因ID
- 255626
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NCBI登录号
- NP_733759
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UniProt
- Q96A08
抗原
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