SMAD6 抗体
Quick Overview for SMAD6 抗体 (ABIN6655220)
抗原
See all SMAD6 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-i15
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Supplier
- Rockland
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原理
- SMAD6 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with Anti-SMAD6 from Human, Mouse, New World monkey, Rat and Sheep based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-SMAD6 Antibody was purified by Protein G chromatography.
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免疫原
- SMAD6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to two internal amino acid sequences in the human SMAD6 protein.
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亚型
- IgG
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应用备注
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Immunohistochemistry_Dilution: 5-10 μg/mL
Western_Blot_Dilution: 1-3 μg/mL
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说明
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Anti-SMAD6 antibody is tested for use in IHC, IF, and WB. Expect a band approximately 53kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))
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别名
- SMAD6
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背景
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Synonyms: MADH6
Background: Anti-SMAD6 antibody detects human SMAD6. SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Anti-SMAD6 antibody is ideal for investigators involved kinase and phosphatase and NFkappaB research.
Gene Name: SMAD6
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基因ID
- 4091
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NCBI登录号
- NP_001136333
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UniProt
- O43541
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途径
- Chromatin Binding
抗原
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