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- 抗原 See all CD28 抗体
- CD28
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD28 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Supplier Product No.
- 200-308-n66
- Supplier
- Rockland
- 原理
- CD28 Phycoerythrin Antibody
- 交叉反应 （详细）
- Reactivity is observed against human CD28, Baboon, Chimpanzee, Cynomolgus, and Rhesus.
- 纯化方法
- Phycoerythrin conjugated CD28 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD28.
- 过滤
- Sterile filtered
- 免疫原
- Anti-CD28 Antibody (Monoclonal) was produced by repeated immunizations with CD28 antigen.
- 克隆位点
- CD28-2
- 亚型
- IgG1 kappa
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells
- 说明
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Anti-CD28 is tested for Flow Cytometry and is useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
- 有效期
- 6 months
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- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 背景
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Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD28
Background: The CD28 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA. The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo.
Gene Name: CD28
- 基因ID
- 940
- NCBI登录号
- NP_001230006
- UniProt
- P10747
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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