Mitochondria 抗体
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- 抗原
- Mitochondria
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 909-301-d79
- Supplier
- Rockland
- 原理
- Mitochondria Antibody
- 交叉反应 (详细)
- Anti-Mitochondria antibody is specific for human mitochondria in all cell types.
- 纯化方法
- The antibody is a protein A purification from concentrated clarified cell culture supernate.
- 免疫原
- Anti-Mitochondria Monoclonal Antibody was produced by repeated immunizations with a human cell homogenate.
- 克隆位点
- 113-1
- 亚型
- IgG1
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- 应用备注
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ELISA_Dilution: 1:10,000 - 1:40,000
Immunohistochemistry_Dilution: 1:800
IF_Microscopy_Dilution: 1:100 - 1:1,000
Western_Blot_Dilution: 1:200 - 1:2,000
- 说明
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Anti-Mitochondria antibody is tested for use in Western Blotting, IHC and IF. Expect ~60kDa in WB. Anti-Anti-Mitochondria antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed cryostat sections. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Mitochondria
- 背景
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Synonyms: mouse anti-Mitochondria Antibody, Human cell marker antibody, xenographic model research antibodies, anti-mitochondria antibody produced in mouse
Background: Mitochondria antibody detects human Mitochondria. Mitochonria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation. In addition, mitochondria have been shown to play a role in apoptosis. This antibody is an excellent marker for human cells in xenographic model research.
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