NEFH 抗体
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北京 101111
Quick Overview for NEFH 抗体 (ABIN6655041)
抗原
See all NEFH 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-901-d82
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Supplier
- Rockland
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原理
- Neurofilament H Antibody
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交叉反应 （详细）
- Anti-NF-H antibody is directed against NF-H.
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纯化方法
- The antibody is a total IgY fraction.
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免疫原
- Anti-Neurofilament NF-H Antibody was produced by repeated immunizations with purified bovine NF-H.
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亚型
- IgG
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anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibodyNEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 兔, 豚鼠, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibodyNEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 821-1020) antibodyNEFH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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IF_Microscopy_Dilution: 1:25000
Western_Blot_Dilution: 1:50000
Other: User Optimized
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说明
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Anti-Neurofilament H antibody is tested for use in Western blotting and IF. Expect a band of approximately 200 kDa in size corresponding to the NF-H proteins in Western blots in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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别名
- Neurofilament H
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背景
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Synonyms: Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Background: NF-H antibody detects Neurofilament H. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-Neurofilament H antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.
Gene Name: NEFH
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基因ID
- 4744
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UniProt
- P12036
抗原
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