Acetylated Lysine 抗体
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北京 101111
Quick Overview for Acetylated Lysine 抗体 (ABIN6655030)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-a97
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Supplier
- Rockland
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原理
- Lysine Acetylated Antibody
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交叉反应 （详细）
- This antibody is known to react with acetylated lysine residues from mammalian and avian sources.
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纯化方法
- This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues.
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过滤
- Sterile filtered
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免疫原
- This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanin (KLH).
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亚型
- IgG1
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anti-Acetylated Lysine antibody
适用: Various Species WB, ELISA, IHC, IP, FACS, FM 宿主: 兔 Polyclonal unconjugated
anti-Acetylated Lysine antibodyWB 宿主: 小鼠 Monoclonal 15G10 unconjugated
anti-Acetylated Lysine antibody适用: Cow, 小鸡 WB, ELISA, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 7F8 unconjugated
anti-Acetylated Lysine antibody适用: Various Species WB 宿主: 兔 Polyclonal unconjugated
anti-Acetylated Lysine (acetylated) antibodyWB, IP, IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: User Optimized
Immunohistochemistry_Dilution: 1:200 - 1:1,000
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized
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说明
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This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 1 μg of antibody is sufficient to detect 20 μg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: See application note.
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Acetylated Lysine
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别名
- Lysine Acetylated
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物质类
- Chemical
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背景
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Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody
Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).
抗原
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