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- 抗原
- DMPO
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunoprecipitation (IP), ELISA, Fluorescence Microscopy (FM)
- 交叉反应
- 所有种类
- 纯化方法
- Anti-DMPO Antibody was purified by Protein G chromatography. DMPO is species independant. This antibody recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA. Cross-reactivity with DMPO from other sources has not been determined. Neuroscience research.
- 免疫原
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Immunogen: DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
Immunogen Type: Other
- 克隆位点
- N1664A
- 亚型
- IgG1
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anti-DMPO antibody
IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A unconjugated
anti-DMPO antibody (HRP)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A HRP
anti-DMPO antibody (PE)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A PE
anti-DMPO antibody (APC)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A APC
anti-DMPO antibody (PerCP)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A PerCP
anti-DMPO antibody (Atto 488)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 488
anti-DMPO antibody (Atto 594)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 594
anti-DMPO antibody (Atto 390)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 390
anti-DMPO antibodyIP, IHC, FM 宿主: 小鼠 Monoclonal N1664A unconjugated
anti-DMPO antibodyIHC, FM 宿主: 小鼠 Monoclonal N1664A unconjugated
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- 应用备注
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Application Note: Anti-DMPO Antibody is suitable for use in Immuno-spin trapping, ELISA, WB, IF microscopy and IP. Expect a band approximately ~90 kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunoprecipitation Dilution: 25 μg
ELISA Dilution: 1-10 μg/mL
IF Microscopy Dilution: 10 μg/mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4
Stabilizer: 50 % (v/v) Glycerol
0.05 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- DMPO
- 物质类
- Chemical
- 背景
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Synonyms: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide
Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
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