DMPO 抗体
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北京 101111
Quick Overview for DMPO 抗体 (ABIN619793)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-DMPO | DMPO nitrone adduct (clone N1664A)
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交叉反应 （详细）
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DMPO (species independent)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- DMPO (species independent)
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纯化方法
- Protein G purified
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免疫原
- 5
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亚型
- IgG1
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anti-DMPO antibody (Atto 390)
IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 390
anti-DMPO antibody (Atto 488)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 488
anti-DMPO antibody (PerCP)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A PerCP
anti-DMPO antibody (PE)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A PE
anti-DMPO antibody (APC)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A APC
anti-DMPO antibody (HRP)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A HRP
anti-DMPO antibody (Atto 594)IP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 594
anti-DMPO antibodyIP, ELISA, WB, IF, ICC, IHC, AA 宿主: 小鼠 Monoclonal N1664A unconjugated
anti-DMPO antibody适用: Adenovirus IP, ELISA, WB, IF, IHC, FM 宿主: 小鼠 Monoclonal N1664A unconjugated
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应用备注
- 1-10 μg/mL (ELISA), 10 μg/mL (ICC), 25 μg (IP), 1-10 μg/mL (WB)
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说明
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Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Total IgG. Protein G purified.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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有效期
- 12 months
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- DMPO
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物质类
- Chemical
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背景
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Background: DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
Additional information: Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
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分子量
- 90 kDa
抗原
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