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pan Keratin 抗体

This anti-pan Keratin antibody is a 兔 单克隆 antibody detecting pan Keratin in IF, FACS, WB 和 IHC (p). Suitable for 人, 大鼠, 小鼠, Cow, 猴, Pig, Frog, 豚鼠 和 山羊.
产品编号 ABIN6654984
发货至: 中国

Quick Overview for pan Keratin 抗体 (ABIN6654984)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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人, 大鼠, 小鼠, Cow, 猴, Pig, Frog, 豚鼠, 山羊

宿主

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克隆类型

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单克隆

标记

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This pan Keratin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

KRT-1877R
  • 纯化方法

    Purified

    纯度

    Protein A affinity chromatography

    免疫原

    A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.

    亚型

    IgG kappa
  • 应用备注

    Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

    1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    pan Keratin (panKRT)

    别名

    Multi Cytokeratin

    背景

    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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