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Recombinant MUC3A 抗体

The 兔 单克隆 anti-MUC3A antibody is suitable to detect MUC3A in samples from 人. It has been validated for IHC (p).
产品编号 ABIN6654970
发货至: 中国
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Quick Overview for Recombinant MUC3A 抗体 (ABIN6654970)

抗原

MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This MUC3A antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

MUC3-2992R
  • 原理

    Recombinant MUC3 Antibody

    纯化方法

    Protein A affinity chromatography

    免疫原

    Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.

    亚型

    IgG, kappa
  • 应用备注

    Optimal dilution of the recombinant MUC3 antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant MUC3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

    别名

    MUC3

    背景

    It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

    UniProt

    Q02505
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