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Ki-67 抗体 (AA 2293-2478)

This anti-Ki-67 antibody is a 小鼠 单克隆 antibody detecting Ki-67 in FACS, IF, ICC 和 IHC (p). Suitable for 人.
产品编号 ABIN6654769
发货至: 中国

Quick Overview for Ki-67 抗体 (AA 2293-2478) (ABIN6654769)

抗原

See all Ki-67 (MKI67) 抗体
Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

适用

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宿主

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小鼠

克隆类型

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  • 86
单克隆

标记

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This Ki-67 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

MKI67-2466
  • 抗原表位

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    AA 2293-2478

    纯化方法

    Purified

    纯度

    Protein G affinity chromatography

    免疫原

    A portion of amino acids 2293-2478 was used as the immunogen for the Ki-67 antibody.

    亚型

    IgG2b kappa
  • 应用备注

    Optimal dilution of the Ki-67 antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 1-2 μg/10^6 cells in 0.1ml,IF/ICC: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Ki-67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

    别名

    Ki67

    背景

    Ki67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki67 index and the histo-pathological grade of neoplasms. Assessment of Ki67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki67 is a good marker of proliferating cell populations.

    途径

    Glycosaminoglycan Metabolic Process
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