POLR2A/RPB1 抗体 (pSer5)
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北京 101111
Quick Overview for POLR2A/RPB1 抗体 (pSer5) (ABIN6654646)
抗原
See all POLR2A/RPB1 (POLR2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer5
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5.
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亚型
- IgG1
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
POLR2A 适用: 人 WB, IF, IHC, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IF, IHC, ELISA, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibodyPOLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibodyPOLR2A 适用: 人 IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (Internal Region) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the recombinant POLR2A antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL,Prediluted IHC only format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the POLR2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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别名
- POLR2A / RNA polymerase II subunit B1
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背景
- RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
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途径
- Regulatory RNA Pathways
抗原
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