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NEFH 抗体

This 兔 单克隆 anti-NEFH antibody (Clone NEFL-H-2324R) specifically detects NEFH in WB 和 IHC (p). The antibody is reactive with 人, 大鼠, 小鼠, 小鸡 和 Pig samples.
产品编号 ABIN6654556
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NEFH 抗体 (ABIN6654556)

抗原

See all NEFH 抗体
NEFH (Neurofilament, Heavy Polypeptide (NEFH))

适用

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人, 大鼠, 小鼠, 小鸡, Pig

宿主

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克隆类型

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单克隆

标记

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This NEFH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

NEFL-H-2324R
  • 纯化方法

    Purified

    纯度

    Protein A affinity

    免疫原

    Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

    亚型

    IgG kappa
  • 应用备注

    The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the recombinant Neurofilament antibody to be titered for optimal performance.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    NEFH (Neurofilament, Heavy Polypeptide (NEFH))

    别名

    Neurofilament (-Heavy) / NF-H / NEFH

    背景

    This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
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