Recombinant BrdU 抗体
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Quick Overview for Recombinant BrdU 抗体 (ABIN6654472)
抗原
See all BrdU 抗体抗体类型
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- BrdU Antibody / Bromodeoxyuridine (azide and preservative free)
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纯化方法
- Protein A affinity chromatography
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免疫原
- BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.
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亚型
- IgG
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.
1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU
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物质类
- Chemical
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背景
- This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
抗原
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