Recombinant Mitochondrial Marker 抗体
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北京 101111
Quick Overview for Recombinant Mitochondrial Marker 抗体 (ABIN6654362)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Mitochondrial Marker Antibody
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纯化方法
- Protein A affinity chromatography
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免疫原
- A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.
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亚型
- IgG, kappa
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anti-Mitochondrial Marker antibody
适用: 人 IF, WB, FACS, IHC 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人, 小鼠, 大鼠 IF, WB, IHC (f), FACS, ISt 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人 IF, WB, IHC (f), Coat, ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mitochondrial Marker antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Mitochondrial Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Mitochondrial Marker
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背景
- This MAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.
抗原
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