Mitochondrial Marker 抗体
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- 抗原
- Mitochondrial Marker
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- This MAb recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
- 无交叉反应
- Bacteria, Insect
- 交叉反应 （详细）
- Shows broad species reactivity. Does not react with insects or bacteria.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Mitochondrial fraction of HeLa cells
- 克隆位点
- MTC719
- 亚型
- IgG1 kappa
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anti-Mitochondrial Marker antibody
适用: 人, 小鼠, 大鼠 IF, IHC (f), WB, FACS, ISt 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人 IF, IHC (f), WB, Coat, ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
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- 应用备注
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Positive Control: HeLa or HepG2 cells. Hepatic carcinomas.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Mitochondrial Marker
- 分子量
- 60kDa
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