ALK 抗体 (AA 200-335)
Quick Overview for ALK 抗体 (AA 200-335) (ABIN6654202)
抗原
See all ALK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 200-335
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纯化方法
- Purified
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纯度
- Protein G affinity
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免疫原
- A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.
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亚型
- IgG2b kappa
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anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 636-666) antibody
ALK 适用: 人 IHC (p), WB 宿主: 兔 Polyclonal RB15425 unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 1591-1620), (C-Term) antibodyALK 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB1513-1514 unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 14-43), (N-Term) antibodyALK 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB1511-1512 unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 636-666) antibodyALK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 1466-1500), (C-Term) antibodyALK 适用: 人 WB 宿主: 兔 Polyclonal RB50500 unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 1517-1550) antibodyALK 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50288 unconjugated
anti-Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (AA 1517-1550) antibodyALK 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant ALK antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))
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别名
- ALK / Anaplastic Lymphoma Kinase
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背景
- The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2,5)(p23,q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5 % -10 % of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.
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途径
- RTK signaling
抗原
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