Reacts with human Hsc70 (Hsp73). Cross-reacts with mouse, rat, rabbit, guinea pig, hamster, monkey, sheep, chicken, bovine, canine and porcine protein.
纯化方法
Purified (protein G)
免疫原
Hsc70 purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.
Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only : WB0.1 μg/mL IP5 μg/mL IHC5 μg/mL ICC5 μg/mL
Positive control: HeLa Heat Shocked Cell Lysate
限制
仅限研究用
缓冲液
PBS pH 7.2, 0.1 mM PMSF in 50 % glycerol.
储存液
PMSF
储存条件
4 °C/-20 °C
储存方法
Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot and store at -20°C.
抗原
Hsc70 (HSPA8)
(Heat Shock 70kDa Protein 8 (HSPA8))
别名
Hsc70 (Hsp73)
背景
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes
Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1 Gene name: HSPA8, HSC70, HSP73, HSPA10