This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.
交叉反应
人
免疫原
Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.
Immunoblotting: use at 1-5 μg/mL. Immunoprecipitation: use at 1-5 μg/mL. Positive controls: MCF-7 cells or recombinant protein.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium which is identical to that used in the assay system.
浓度
Lot specific
缓冲液
PBS, pH 7.4
储存条件
-80 °C
储存方法
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
抗原
LIG1
(Ligase I, DNA, ATP-Dependent (LIG1))
别名
DNA ligase 1
背景
DNA ligase 1,DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,Nucleus.,EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1