Immunoblotting: Use at 0.5-2 μg/mL. A band of ~80 kDa is detected. Detection of Mre11 in 30 μg of whole cell extracts. Immunofluorescence: use at 1-5 μg/mL. Detection of Mre11 in HeLa cells fixed in 4 % paraformaldehyde for 15 minutes. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium which is identical to that used in the assay system.
浓度
Lot specific
缓冲液
PBS, pH 7.4
储存条件
-20 °C,-80 °C
储存方法
This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.
抗原
Mre11 (MRE11A)
(MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))
别名
MRE11
背景
Double-strand break repair protein MRE11,Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}.,Nucleus, Chromosome, telomere, Chromosome,EC 3.1.-.-, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A