NONO 抗体 (N-Term)
Quick Overview for NONO 抗体 (N-Term) (ABIN629888)
抗原
See all NONO 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- NONO antibody was raised against the N terminal of NONO
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纯化方法
- Purified
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免疫原
- NONO antibody was raised using the N terminal of NONO corresponding to a region with amino acids KQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLK
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应用备注
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WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator. -
说明
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NONO Blocking Peptide, catalog no. 33R-4610, is also available for use as a blocking control in assays to test for specificity of this NONO antibody
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NONO antibody in PBS
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浓度
- Lot specific
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缓冲液
- PBS
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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别名
- NONO
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背景
- NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
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分子量
- 52 kDa (MW of target protein)
抗原
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