CD63 抗体
Quick Overview for CD63 抗体 (ABIN6297773)
抗原
See all CD63 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Mouse anti-Human/Mouse CD63 Antibody [Sodium Azide Free]
-
特异性
- Cell Surface & Cytoplasmic
-
纯化方法
- The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this anti-CD63 antibody.
-
免疫原
- The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this anti-CD63 antibody.
-
-
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 2-4 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
缓冲液
- In 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
-
-
- CD63
-
别名
- CD63 antigen
-
背景
-
Target Description: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Gene Symbol: CD63///MLA1///TSPAN30
-
基因ID
- 967
-
UniProt
- P08962
抗原
-