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GLG1 抗体

GLG1 适用: 人 WB, IHC, ICC, FACS, IF 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN6297737
发货至: 中国
  • 抗原 See all GLG1 抗体
    GLG1 (Golgi Glycoprotein 1 (GLG1))
    适用
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    宿主
    • 22
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    小鼠
    克隆类型
    • 23
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    单克隆
    标记
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    This GLG1 antibody is un-conjugated
    应用范围
    • 30
    • 18
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    Mouse anti-Human Golgi Marker Antibody [Sodium Azide Free]
    特异性
    Golgi complex in cytoplasm
    纯化方法
    The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.
    免疫原
    The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.
    Top Product
    Discover our top product GLG1 Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 0.5-1 μg/mL
    Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
    Western blot: 0.5-1.0 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    限制
    仅限研究用
  • 缓冲液
    In 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原
    GLG1 (Golgi Glycoprotein 1 (GLG1))
    别名
    Golgi apparatus protein 1 (GLG1 产品)
    别名
    AI593353 antibody, AW537898 antibody, CFR antibody, CFR-1 antibody, ESL-1 antibody, MG-160 antibody, MG160 antibody, Selel antibody, ESL1 antibody, LTCP-1 antibody, golgi glycoprotein 1 antibody, Golgi apparatus protein 1 antibody, golgi apparatus protein 1 antibody, GLG1 antibody, LOC100175586 antibody, Glg1 antibody
    背景

    Target Description: This mAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This mAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

    Gene Symbol: GLG1///CFR1///ESL1///MG160

    基因ID
    2734
    UniProt
    Q92896
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