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Golgi 抗体

适用: 人 FACS, IF, ICC, WB, IHC 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN6297711
发货至: 中国
  • 抗原
    Golgi
    适用
    宿主
    • 5
    • 2
    小鼠
    克隆类型
    • 7
    单克隆
    标记
    • 7
    非结合性
    应用范围
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blotting (WB), Immunohistochemistry (IHC)
    原理
    Mouse anti-Human Golgi Antibody [Sodium Azide Free]
    特异性
    Golgi complex in cytoplasm
    纯化方法
    SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
    免疫原
    SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
  • 应用备注
    Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
    Immunofluorescence: 0.5-1 μg/mL
    Western blot: 0.5-1 μg/mL,ICC (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
    限制
    仅限研究用
  • 缓冲液
    In 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原
    Golgi
    背景

    Target Description: This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.

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