Golgi 抗体
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北京 101111
Quick Overview for Golgi 抗体 (ABIN7880911)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Golgi Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
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亚型
- IgG1, kappa
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anti-Golgi antibody
Carrier-free 适用: 人 IHC (p), FACS, IF, ICC, WB 宿主: 小鼠 Monoclonal AE-6 unconjugated
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应用备注
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The optimal dilution of the anti-Golgi antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Golgi
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背景
- This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
抗原
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