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CD20 抗体

This anti-CD20 antibody is a 小鼠 单克隆 antibody detecting CD20 in FACS, IF 和 Func. Suitable for 人.
产品编号 ABIN6297696
发货至: 中国

Quick Overview for CD20 抗体 (ABIN6297696)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD20 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
  • 原理

    Mouse anti-Human CD20 Antibody [Sodium Azide Free]

    特异性

    Predominantly cell surface with some cytoplasmic

    纯化方法

    Stimulated human leukocytes were used as the immunogen for this CD20 antibody.

    免疫原

    Stimulated human leukocytes were used as the immunogen for this CD20 antibody.
  • 应用备注

    FACS: 0.5-1 μg/million cells
    IF: 0.5-1 μg/mL
    Functional Testing (Order Ab without BSA & Azide),

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS ( pH 7.4), BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    B-lymphocyte antigen CD20

    背景

    Target Description: This antibody recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

    Gene Symbol: MS4A1///CD20

    基因ID

    931

    UniProt

    P11836
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