CD176 抗体
-
- 抗原 See all CD176 products
- CD176 (Thomsen Friedenreich Antigen (CD176))
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD176 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- Mouse anti-Human/Mouse/Rat Thomsen-Friedenreich Antigen Antibody [Sodium Azide Free]
- 特异性
- Cell surface
- 纯化方法
- Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.
- 免疫原
- Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.
-
anti-Thomsen Friedenreich Antigen (CD176) antibody
CD176 适用: 人, 小鼠, 大鼠 IHC, IF, StM 宿主: 小鼠 Monoclonal A78-G-A7 unconjugated
-
- 应用备注
-
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT - 限制
- 仅限研究用
-
- 缓冲液
- In 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
-
- 抗原
- CD176 (Thomsen Friedenreich Antigen (CD176))
- 别名
- Thomsen-Friedenreich Antigen (CD176 产品)
- 背景
-
Target Description: Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
-
