CD34 抗体
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北京 101111
Quick Overview for CD34 抗体 (ABIN6295964)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Cynomolgus Monkey/Rhesus Monkey CD34 Antibody [Sodium Azide Free]
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特异性
- Cell surface
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纯化方法
- A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.
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免疫原
- A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.
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应用备注
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FACS: use antibody at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS ( pH 7.4), BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD34
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别名
- Hematopoietic progenitor cell antigen CD34
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背景
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Target Description: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi 's sarcoma, but with a lower specificity.
Gene Symbol: CD34
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基因ID
- 947
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UniProt
- P28906
抗原
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