Keratin 10 抗体
Quick Overview for Keratin 10 抗体 (ABIN6295934)
抗原
See all Keratin 10 (KRT10) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Cat Cytokeratin 10 + 13 Antibody [Sodium Azide Free]
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特异性
- Cytoplasmic
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纯化方法
- A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
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免疫原
- A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Keratin 10 (KRT10)
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别名
- Keratin, type I cytoskeletal 10
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背景
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Target Description: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Gene Symbol: KRT10///KPP///KRT13
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UniProt
- P13645, P13646
抗原
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