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Keratin 10 抗体

This anti-Keratin 10 antibody is a 小鼠 单克隆 antibody detecting Keratin 10 in IHC, WB, FACS 和 IF. Suitable for 人 和 Cat.
产品编号 ABIN6295934
发货至: 中国

Quick Overview for Keratin 10 抗体 (ABIN6295934)

抗原

See all Keratin 10 (KRT10) 抗体
Keratin 10 (KRT10)

适用

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人, Cat

宿主

  • 103
  • 94
  • 2
小鼠

克隆类型

  • 136
  • 63
单克隆

标记

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  • 10
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This Keratin 10 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
  • 原理

    Mouse anti-Human/Cat Cytokeratin 10 + 13 Antibody [Sodium Azide Free]

    特异性

    Cytoplasmic

    纯化方法

    A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.

    免疫原

    A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 0.5-1 μg/mL
    Western blot: 0.25-0.5 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    Keratin 10 (KRT10)

    别名

    Keratin, type I cytoskeletal 10

    背景

    Target Description: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

    Gene Symbol: KRT10///KPP///KRT13

    UniProt

    P13645, P13646
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