KIT 抗体 (AA 100-300)
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Quick Overview for KIT 抗体 (AA 100-300) (ABIN6294267)
抗原
See all KIT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-300
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原理
- Mouse anti-Human c-Kit Antibody [Sodium Azide Free]
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特异性
- Cell surface and cytoplasmic
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纯化方法
- A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.
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免疫原
- A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: (0.5-1 μg/mL for 2 hours at RT)
Immunohistochemistry (Frozen Only): 2-4 μg/mL for 30 min at RT -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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别名
- Mast/stem cell growth factor receptor Kit
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背景
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Target Description: This mAb recognizes a protein of 145 kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Gene Symbol: KIT///SCFR
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基因ID
- 3815
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UniProt
- P10721
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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