FBXO5 抗体 (AA 1-250)
Quick Overview for FBXO5 抗体 (AA 1-250) (ABIN6294242)
抗原
See all FBXO5 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-250
-
原理
- Mouse anti-Human EMI1 Antibody [Sodium Azide Free]
-
特异性
- Nuclear
-
纯化方法
- A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.
-
免疫原
- A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.
-
-
anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibody
FBXO5 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人, 犬, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5H7 unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 326-375) antibodyFBXO5 适用: 人, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibodyFBXO5 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
缓冲液
- In 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
-
-
- FBXO5 (F-Box Protein 5 (FBXO5))
-
别名
- F-box only protein 5
-
背景
-
Target Description: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Gene Symbol: FBXO5///EMI1///FBX5
-
基因ID
- 26271
-
UniProt
- Q9UKT4
-
途径
- Mitotic G1-G1/S Phases
抗原
-
