RNF20 抗体 (Internal Region)
Quick Overview for RNF20 抗体 (Internal Region) (ABIN6264788)
抗原
See all RNF20 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- RNF20 Antibody detects endogenous levels of total RNF20.
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预测反应
- Pig,Bovine,Horse,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human RNF20, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Ring Finger Protein 20 (RNF20) (AA 1-975) antibody
RNF20 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) antibodyRNF20 适用: 人 WB 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 177-276) antibodyRNF20 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 1-975) antibodyRNF20 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 177-276) antibodyRNF20 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E4 unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 576-625) antibodyRNF20 适用: 人, 小鼠, 犬, Cow, 豚鼠, Pig, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 108-157) antibodyRNF20 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) (AA 1-260) antibodyRNF20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 20 (RNF20) (N-Term) antibodyRNF20 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- RNF20 (Ring Finger Protein 20 (RNF20))
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别名
- RNF20
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背景
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Description: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Gene: RNF20
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分子量
- 114kDa
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基因ID
- 56254
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UniProt
- Q5VTR2
抗原
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