PYCARD 抗体 (N-Term)
(1 reference)-
- 抗原 See all PYCARD 抗体
- PYCARD (PYD and CARD Domain Containing (PYCARD))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PYCARD antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- PYCARD Antibody detects endogenous levels of total PYCARD.
- 预测反应
- Bovine,Horse,Sheep,Rabbit
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human PYCARD, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product PYCARD Primary Antibody
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anti-PYD and CARD Domain Containing (PYCARD) (AA 50-195) antibody
PYCARD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (AA 50-195) antibodyPYCARD 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibodyVerified PYCARD 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) antibodyPYCARD 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (AA 1-120) antibodyPYCARD 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 1C3D3 unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (Internal Region) antibodyVerified PYCARD 适用: 人, 小鼠, 大鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (AA 1-149) antibodyPYCARD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (AA 1-195) antibodyPYCARD 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) antibodyPYCARD 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-PYD and CARD Domain Containing (PYCARD) (AA 1-195) antibodyPYCARD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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: "Ozone protects the rat lung from ischemia-reperfusion injury by attenuating NLRP3-mediated inflammation, enhancing Nrf2 antioxidant activity and inhibiting apoptosis." in: European journal of pharmacology, Vol. 835, pp. 82-93, (2018) (PubMed).
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: "Ozone protects the rat lung from ischemia-reperfusion injury by attenuating NLRP3-mediated inflammation, enhancing Nrf2 antioxidant activity and inhibiting apoptosis." in: European journal of pharmacology, Vol. 835, pp. 82-93, (2018) (PubMed).
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- 抗原
- PYCARD (PYD and CARD Domain Containing (PYCARD))
- 别名
- PYCARD (PYCARD 产品)
- 别名
- ASC antibody, CARD5 antibody, TMS antibody, TMS-1 antibody, TMS1 antibody, 9130417A21Rik antibody, Asc antibody, TNS1 antibody, masc antibody, asc1 antibody, zAsc antibody, zgc:109874 antibody, PYD and CARD domain containing antibody, PYCARD antibody, Pycard antibody, pycard antibody
- 背景
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Description: Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2, the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome, this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590).
Gene: PYCARD
- 分子量
- 25kDa
- 基因ID
- 29108
- UniProt
- Q9ULZ3
- 途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
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