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PYCARD 抗体 (C-Term)

The 山羊 多克隆 anti-PYCARD antibody (ABIN184674) specifically detects PYCARD in WB, ELISA 和 IHC. The antibody is reactive with 人 samples.
产品编号 ABIN184674
发货至: 中国
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中国
北京 101111
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Room 801-803
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Quick Overview for PYCARD 抗体 (C-Term) (ABIN184674)

抗原

See all PYCARD 抗体
PYCARD (PYD and CARD Domain Containing (PYCARD))

适用

  • 77
  • 35
  • 24
  • 1
  • 1
  • 1
人

宿主

  • 92
  • 8
  • 3
山羊

克隆类型

  • 80
  • 23
多克隆

标记

  • 41
  • 9
  • 5
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
This PYCARD antibody is un-conjugated

应用范围

  • 84
  • 58
  • 45
  • 43
  • 43
  • 35
  • 8
  • 3
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

    • 18
    • 13
    • 12
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    ASC / TMS1

    序列

    RESQSYLVED LERS

    特异性

    This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).

    交叉反应

    人

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6 μg/mL.

    Western Blot: Approx 19 kDa band observed in lysates of cell line HeLa (calculated MW of 20.0 kDa according to NP_660183.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    PYCARD (PYD and CARD Domain Containing (PYCARD))

    别名

    PYCARD

    背景

    PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1

    基因ID

    29108

    NCBI登录号

    NP_037390, NP_660183

    途径

    Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
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