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PML 抗体 (N-Term)

Name: PML 抗体 (N-Term)
SKU: ABIN6264315
Availability: OutOfStock

This anti-PML antibody is a 兔多克隆 antibody detecting PML in WB, IHC, IF, ELISA 和 ICC. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 1 publication.

Western blot analysis of HeLa lysate using PML antibody. (PML 抗体 (N-Term))

ABIN6276587 at 1/100 staining Human kidney tissue by IHC-P. (PML 抗体 (N-Term))

IHC

IF/ICC

F6318 staining HEPG2 cells by ICC/IF. (PML 抗体 (N-Term))

IF/ICC

4 images

(1 reference)

产品编号 ABIN6264315

发货至: 中国

Datasheet as PDF

Quick Overview for PML 抗体 (N-Term) (ABIN6264315)

抗原

See all PML 抗体

PML (Promyelocytic Leukemia (PML))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PML antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Discover our top product PML Primary Antibody

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N-Term

特异性

PML Antibody detects endogenous levels of total PML.

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human PML, corresponding to a region within N-terminal amino acids.

亚型

IgG
anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody
PML 适用: 人 WB, IF, ELISA, PLA 宿主: 小鼠 Monoclonal 1D12 unconjugated

anti-Promyelocytic Leukemia (PML) (AA 470-499), (Sumoylation Site) antibody
PML 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (AA 300-600) antibody
PML 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (C-Term) antibody
PML 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody
PML 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2B10 unconjugated

anti-Promyelocytic Leukemia (PML) (C-Term) antibody
PML 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (AA 470-499), (Sumoylation Site) antibody
PML 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB2570 unconjugated

anti-Promyelocytic Leukemia (PML) (N-Term) antibody
PML 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (AA 588-637) antibody
PML 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Promyelocytic Leukemia (PML) (AA 59-239) antibody
PML 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Wu, Shao, Zhou, Sun, Wang, Yan, Liu, Wu, Ye, Huang, Zhou et al.: "Autophagy and Ubiquitin-Mediated Proteolytic Degradation of PML/Rarα Fusion Protein in Matrine-Induced Differentiation Sensitivity Recovery of ATRA-Resistant APL (NB4-LR1) Cells: in Vitro and in Vivo ..." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 48, Issue 6, pp. 2286-2301, (2018) (PubMed).
抗原

PML (Promyelocytic Leukemia (PML))

别名

PML

背景

Description: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function. Isoform PML-6 inhibits specifically the activity of the tetrameric form of PKM. The nuclear isoforms (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Isoform PML-2 is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration.
Gene: PML

分子量

98kDa

基因ID

5371

UniProt

P29590

途径

p53 Pathway, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus