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PKC beta 抗体 (C-Term)

Name: PKC beta 抗体 (C-Term)
SKU: ABIN6264249
Availability: OutOfStock

This anti-PKC beta antibody is a 兔多克隆 antibody detecting PKC beta in WB, ELISA, IF 和 ICC. Suitable for 人, 大鼠和小鼠.

Western blot analysis of PKCB expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (PKC beta 抗体 (C-Term))

IF/ICC

2 images

产品编号 ABIN6264249

发货至: 中国

Quick Overview for PKC beta 抗体 (C-Term) (ABIN6264249)

抗原

See all PKC beta (PRKCB) 抗体

PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

适用

人, 大鼠, 小鼠

宿主

兔

克隆类型

多克隆

标记

This PKC beta antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product PRKCB Primary Antibody

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C-Term

特异性

PKC beta 1 Antibody detects endogenous levels of total PKC beta 1.

预测反应

Bovine,Sheep,Rabbit

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human PKC beta 1, corresponding to a region within C-terminal amino acids.

亚型

IgG
anti-Protein Kinase C, beta (PRKCB) (AA 1-270) antibody
PRKCB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 220-340) antibody
PRKCB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (pThr500) antibody
PRKCB 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 517-643) antibody
PRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (pThr641) antibody
PRKCB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (Internal Region) antibody
Verified PRKCB 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 642-673) antibody
PRKCB 适用: 人, 大鼠, 小鼠, 灵长类 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 601-673) antibody
PRKCB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 261-341) antibody
PRKCB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (AA 1-671) antibody
PRKCB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

别名

PRKCB

背景

Description: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Gene: PRKCB

分子量

77kDa

基因ID

5579

UniProt

P05771

途径

WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling